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incucyte zoom sartorius n a beckman cytoflex analyzer beckman coulter n a expert gtx tm gmp electroporator maxcyte n a oc  (Sartorius AG)
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Sartorius AG incucyte zoom sartorius n a beckman cytoflex analyzer beckman coulter n a expert gtx tm gmp electroporator maxcyte n a oc
Incucyte Zoom Sartorius N A Beckman Cytoflex Analyzer Beckman Coulter N A Expert Gtx Tm Gmp Electroporator Maxcyte N A Oc, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
incucyte zoom sartorius n a beckman cytoflex analyzer beckman coulter n a expert gtx tm gmp electroporator maxcyte n a oc - by Bioz Stars, 2026-06
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electroporation instrument  (Bio-Rad)
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Bio-Rad electroporation instrument
Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Electroporation Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electroporation instrument/product/Bio-Rad
Average 97 stars, based on 1 article reviews
electroporation instrument - by Bioz Stars, 2026-06
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electroporation instrument  (Ningbo Scientz Biotechnology)
86
Ningbo Scientz Biotechnology electroporation instrument
Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Electroporation Instrument, supplied by Ningbo Scientz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electroporation instrument/product/Ningbo Scientz Biotechnology
Average 86 stars, based on 1 article reviews
electroporation instrument - by Bioz Stars, 2026-06
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bio rad micropulser electroporator instrument  (Bio-Rad)
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Bio-Rad bio rad micropulser electroporator instrument
Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Bio Rad Micropulser Electroporator Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
bio rad micropulser electroporator instrument - by Bioz Stars, 2026-06
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bio rad micropulser electroporator 400 instrument  (Bio-Rad)
96
Bio-Rad bio rad micropulser electroporator 400 instrument
Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Bio Rad Micropulser Electroporator 400 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bio rad micropulser electroporator 400 instrument/product/Bio-Rad
Average 96 stars, based on 1 article reviews
bio rad micropulser electroporator 400 instrument - by Bioz Stars, 2026-06
96/100 stars
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eppendorf electroporation instrument  (Eppendorf AG)
95
Eppendorf AG eppendorf electroporation instrument
Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Eppendorf Electroporation Instrument, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eppendorf electroporation instrument - by Bioz Stars, 2026-06
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gene pulser instrument  (Bio-Rad)
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Bio-Rad gene pulser instrument
Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Gene Pulser Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene pulser instrument/product/Bio-Rad
Average 96 stars, based on 1 article reviews
gene pulser instrument - by Bioz Stars, 2026-06
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inovio s electroporation instruments  (Inovio Inc)
86
Inovio Inc inovio s electroporation instruments
Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Inovio S Electroporation Instruments, supplied by Inovio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inovio s electroporation instruments/product/Inovio Inc
Average 86 stars, based on 1 article reviews
inovio s electroporation instruments - by Bioz Stars, 2026-06
86/100 stars
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inovio s medpulser electroporation instrument  (Inovio Inc)
86
Inovio Inc inovio s medpulser electroporation instrument
Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Inovio S Medpulser Electroporation Instrument, supplied by Inovio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inovio s medpulser electroporation instrument/product/Inovio Inc
Average 86 stars, based on 1 article reviews
inovio s medpulser electroporation instrument - by Bioz Stars, 2026-06
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Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by electroporation; Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.

Journal: STAR Protocols

Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

doi: 10.1016/j.xpro.2026.104353

Figure Lengend Snippet: Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by electroporation; Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.

Article Snippet: Electroporation instrument , BIO-RAD , Gene Pulser Xcell.

Techniques: Plasmid Preparation, Transformation Assay, Marker, Amplification, Electroporation, Homologous Recombination, Selection, Mutagenesis

Identification of the interaction between CRP and YdeH using the BACTH system Both crp and ydeH were cloned and inserted into the pUT18C and pKNT25 vectors, respectively, and co-transformed into the BTH101 strain by electroporation. The positive clones were spotted together with the negative control and positive control on the colour plate. Finally, the colour development of the plate was checked. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.

Journal: STAR Protocols

Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

doi: 10.1016/j.xpro.2026.104353

Figure Lengend Snippet: Identification of the interaction between CRP and YdeH using the BACTH system Both crp and ydeH were cloned and inserted into the pUT18C and pKNT25 vectors, respectively, and co-transformed into the BTH101 strain by electroporation. The positive clones were spotted together with the negative control and positive control on the colour plate. Finally, the colour development of the plate was checked. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.

Article Snippet: Electroporation instrument , BIO-RAD , Gene Pulser Xcell.

Techniques: Clone Assay, Transformation Assay, Electroporation, Negative Control, Positive Control